兔晶体上皮细胞的体外培养

In vitro culture of rabbit lens epithelial cells

目的建立兔晶体上皮细胞体外培养的简便方法并观察原代和传代细胞的生物学特征和组织学变化。方法将家兔晶体前囊经消化、离心,以获取分散的晶体上皮细胞,在MEM培养基中培养2周,采用倒置显微镜观察活体细胞的增殖活动和形态学变化。结果兔晶体上皮细胞在体外培养时可以存活并传代,晶体上皮细胞在体外的增殖力与兔龄呈负相关性。结论本方法简便,有效,可用于实验研究。

Objective To setup an easier procedure of culturing rabbit lens epithelial cells in vitro and to observe the biological characteristics and histological changes of both primary and passage cells. Methods Lens epithelial cells obtained from the rabbit anterior capsule by digesting and centrifuging are cultured in the MEM for two weeks, and the proliferative behavior and morphological changs were observed under invert contrast microscope. Results Cultured rabbit lens epithelial cell line in vitro was alive and passed for several generations. But the activity of later generations was limited and related to the rabbits＇age. Conclusion This method is very easy and effective, and can be used in experimental study.