HPLC检测南海蕨藻中的蕨藻红素

Foundation of method of caulerpin in fern algae of South China Sea measured by HPLC

建立南海大型经济海藻总状蕨藻的主要有效成分蕨藻红素的高效液相色谱（HPLC）定量分析检测方法。取干燥蕨藻加体积分数95％乙醇80℃加热回流3次，回流时间为3h，固液比为1：20．减压浓缩得蕨藻乙醇提取物浸膏，精密称取浸膏，甲醇10m1定容．采用Discovery C_18（4．6mm×150mm，5μm）反相柱，DAD检测器。检测波长315nm，进样20μL，流动相为甲醇和水（质量分数1‰三氟乙酸）梯度洗脱，流速1．0mL／min。蕨藻红素在1．006～10．06μg范围内呈良好的线性关系，r=0．9999，平均回收率为98．0％，RSD=0．92％。测得所采集的干燥总状蕨藻中蕨藻红素的质量分数为19．93mg／g。该方法灵敏、可靠、准确性高，可用于同属海藻的蕨藻红素的定量分析检测。

A quantitative analysis meathod for the determination of is established in this paper eaulerpin in Fern Algae of South China Sea by High Performance Liquid Chromatography （ HPLC ）. Drying Caulerpa was extracted with 95 % ethanol by heating cireumfluenee in 80℃ for 3 times and the refluxing time of 3 h, and the ratio 1 ： 10. Then the ethanol extract was got by vaeeuum concentration and dissolved with 10 ml methanol. HPLC was perfomed on Discovery C_18 （4.6 mm×150mm,5 μm）, DAD detetor. The detection wavelengh was 315 nm, the mobile phase was methanol and water（ quality score 1‰TFA）, velocity of flow was 1.0 mL/min, injection volume was 20μL. The content of eaulerpin showed good linearity in the range of 1. 006 - 10.06μg, r =0. 9999. The average recovery was 98.0% , RSD =0. 92%. The results showed that per gram dry Fern Algae contained 19. 93 mg caulerpin. The quantitative analysis meathod is sensitive, reliable and selective, and can be used for the detective of culerpin existing in algae.