脉冲磁场对颈动脉内膜切除术后血管内膜血小板衍生生长因子表达的影响

Effect of Pulsed Magnetic Field Therapy on Expression of Platelet-Derived Growth Factor-A after Carotid Endarterectomy

目的观察不同强度脉冲磁场对颈动脉粥样硬化家兔模型颈动脉内膜切除术(carotid endarterectomy,CEA)后血管内膜血小板衍生生长因子-A(platelet-derived growth factor,PDGF-A)表达的影响。方法家兔50只,制作家兔颈动脉粥样硬化模型。高脂饲料喂养2月后行CEA手术。术后将实验动物随机分为对照组、0.6T治疗组和1.0T治疗组。在CEA术后1个月、2个月及3个月使用免疫组化方法观察病变血管内膜PDGF-A表达。结果CEA术后2个月,治疗组PDGF-A水平低于对照组(P<0.05);术后3个月,1.0T治疗组PDGF-A表达水平低于对照组(P<0.01)和0.6T治疗组(P<0.05),0.6T治疗组低于对照组(P<0.05)。结论适当剂量脉冲磁场能抑制CEA术后颈动脉内膜PDGF-A的表达,对CEA术后再狭窄的发生有一定的防治作用。

Objective To investigate the effect of different dosage of pulsed magnetic field on expression of platelet-derived growth factor-A （PDGF-A） in rabbits with carotid atheroselerosis after carotid endarterectomy （CEA）. Methods Carotid atheroscle- rosis were modeled in 50 New Zealand rabbits with air-drying lesion combined with high fat feed. All animals were treated with double side carotid endarterectomy after 2 months, and were randomly divided 3 groups： 0.6 T group, 1.0 T group and the blank control. The expression of platelet-derived growth factor-A were detected with immunohistochemistry 1 month, 2 months and 3 months after CEA. Results The expression of PDGF-A in treated groups were lower significantly than that in controls （P〈0.05） 2 month after CEA, and it was the lowest in 1.0 T group, lower in 0.6 T group 3 month after CEA. Conclusion The pulsed magnetic field therapy can decrease the expression of PDGF-A, which might be helpful to prevent the carotid from restenosis.