电刺激迷走神经对感染性休克大鼠血浆肿瘤坏死因子α、一氧化氮合酶及一氧化氮的影响

Effects of Electrical Stimulation of Vagus Nerve on Plasma Tumor Necrosis Factor α,Nitric Oxide Synthases and Nitric Oxide in Septic Shock Rats

目的研究电刺激迷走神经对感染性休克大鼠血浆肿瘤坏死因子α(TNF-α)、一氧化氮合酶(NOS)及一氧化氮(NO)水平的影响。方法成年雄性SD大鼠40只,采用盲肠结扎穿孔法(CLP)复制感染性休克模型,随机分为5组:假CLP组、CLP组、迷切组、电刺激左侧迷走神经组、电刺激右侧迷走神经组。各组动物均行颈总动脉置管连续监测平均动脉压,ELISA法检测血浆TNF-α,生化法检测血浆中NOS活性和NO水平。结果CLP组术后平均动脉血压进行性下降,2h时血浆TNF-α、NOS及NO水平显著升高;与CLP组比较,电刺激组动物平均动脉压下降幅度减轻,血浆TNF-α、NOS及NO水平显著降低。结论电刺激左、右迷走神经均可能缓解CLP致感染性休克大鼠的进行性血压下降,降低血浆TNF-α、NOS及NO水平,有助于抗休克。

Objective To study the effect of electrical stimulation of vagus nerve on inflammatory response in septic shock rats. Methods SD rats were randomly divided into 5 groups.. Group Ⅰ was the sham group, group Ⅱ with the cecal ligation and puncture （CLP） and the vagus nerve were isolated but not transected, group Ⅲ with bilateral cervical vagotomy following CLP, group IV with bilateral cervical vagotomy after CLP and the left vagus nerve trunks were stimulated with bipolar electrodes, group V with bilateral cervical vagotomy after CLP and the right vagus nerve trunks were stimulated. The common carotid artery pressure was monitored, and the plasma tumor necrosis factor a （TNF-α）, nitric oxide synthases （NOS） and nitric oxide （NO） were measured 2 h after stimulation. Results The mean arterial blood pressure （MAP） gradually decreased and the concentration of plasma TNF-α, NOS and NO significantly increased after CLP. Electrical stimulation of the left and right vagus nerve significantly increased the MAP and decreased the plasma TNF-α, NOS and NO levels. Conclusion Direct electrical stimulation of the left and right vagus nerve can significantly improve the blood pressure and reduced plasma TNF-α, NOS and NO levels during septic shock, which may play a role in anti-shock in rats.