Survivin在卵巢癌组织中的表达及其与P53表达相关性的研究

Expression of survivin and its relationship with expression of p53 in ovarian carcinoma

目的探讨survivin在卵巢癌组织中的表达及其与p53表达的相关性。方法应用S-P免疫组化染色法检测survivin、p53蛋白在48例卵巢癌中的表达,同时选8例卵巢交界性肿瘤及13例良性肿瘤及非赘生性囊肿作为对照。结果survivin在卵巢癌组表达率为87.5%,明显高于在良性肿瘤及非赘生性囊肿的表达率15.4%,P<0.01。survivin基因阳性表达与患者年龄,肿瘤组织来源,及卵巢癌临床分期无相关性。p53在卵巢癌中表达率为64.6%,良性卵巢瘤及非赘生性囊肿表达率为23.1%,两者之间差异具有显著性,P<0.05。p53蛋白表达阳性及阴性卵巢癌组织中,survivin表达阳性率分别为96.8%、70.6%,两者之间具有明显的正相关,P<0.05。结论survivin引起的细胞凋亡抑制,在卵巢癌的发生、发展中起一定作用,survivin与p53之间可能具有协同作用。

[Objective] To study the expression of survivin and its relationship with expression of p53 in ovarian carcinoma. [Methods] Using streptavidin-biotin peroxidase （SP） method, we examined the expression of survivin, p53 proteins in 13 ovarian benign tumors, 8 ovarian bordline tumors and 48 ovarian carcinomas. [Results] Survivin was expressed in 42 of 48 （87.5%） cases of ovarian carcinomas. In contrast, survivin was expressed in 2 of 13 （15.4%） cases of ovarian benign tumors （P 〈0.01）. Survivin positive cases were associated with p53 expression （30/ 31, 96.8% versus 12 of 17 cases, P 〈0.05）. [Conclusion] Apoptosis inhibition by survivin may participate in the onset and progression of ovarian carcinoma.