MODS小鼠脾脏高迁移率族蛋白B1表达变化与树突状细胞功能的关系

Change of splenic HMGB1 expression influences dendritic cell activity of spleen in mice with MODS

目的探讨多器官功能障碍综合症发生、发展过程中脾脏树突状细胞功能状态与HMGB1表达变化的关系。方法腹腔注射酵母多糖复制小鼠MODS模型,检测MODS病程中小鼠脾脏HMGB1表达水平及树突状细胞数量和功能相关分子CD205、CD86表达的变化。结果正常小鼠脾脏有较低水平HMGB1表达,伤后3h表达增加(P<0.05);伤后24～48h表达量达峰值,随后减少;伤后5～7dHMGB1表达降至接近正常水平;伤后10～12dHMGB1表达再次增多(P<0.05)。脾脏HMGB1 mRNA与蛋白表达变化趋势基本一致,在伤后24～48h和伤后10～12d形成两次高峰(P<0.01)。在MODS病程前期,脾脏树突状细胞数量及活性与HMGB1含量呈同向变化,但在病程晚期两者变化规律不同。结论MODS小鼠脾脏HMGB1上调表达可能参与调节树突状细胞的功能状态和免疫活性,从而影响MODS的发生和发展。

[Objective] To explore the effect of high mobility group protein box 1 （HMGB1） expression on the activity of dendritic cells in the spleen of mice with multiple organ dysfunction syndrome （MODS）. [Methods] MODS model was replicated by injecting zymosan into peritoneal cavity of mice. The expression levels of HMGB1, the number of dendritic cells and CD306 and CD86 on the cells, and apoptosis rate in the spleen were detected. [Results] There is low level of HMGB1 expressed in the spleen of normal mice. At 8-12 hours after blunted, HMGBI expression was increased, and reached the highest level on 1-2 days, then decreased on 5 days, but increased on 10-12 days （P 〈0.05） again. The number and the activity of dendritic cells were changed positively according to the amount of HMGB1 expression at the early stage of MODS, but changed reversely at the late stage. [Conclusion] In mice with MODS, change of the splenic HMGB1 content might be involved in regulating functional status and immunological activity of the splenic dendritic cells, which influence the development of MODS.