CS/TPP纳米微胶囊的制备及其载药性能

Preparation and Property of Chitosan/Sodium Tripolyphosphate Microcapsules as Drug Carrier

利用离子凝胶法,以三聚磷酸钠(TPP)为交联剂,由壳聚糖(CS)制备了CS/TPP纳米微胶囊.用红外光谱仪、扫描电镜和粒径分析仪进行了表征,并以牛血清蛋白(BSA)作为模型药物,考察了所制备的CS/TPP纳米微胶囊的包载和缓释性能.结果表明,CS/TPP纳米微胶囊的红外光谱相对于CS和TPP的红外光谱发生了很大变化,说明CS和TPP通过正负电荷吸引聚合成囊;粒径分析表明,离子凝胶法可以得到粒径约430 nm的均匀分散的壳聚糖纳米微胶囊,经冷冻干燥后粒径变为300 nm左右;微胶囊包封率最高可达79.74%,模型药物的持续释放时间可达7 d以上.

Chitosan/sodium tripolyphosphate（CS/TPP） nano-sized microcapsules were prepared with ionic gelation of CS with TPP. The obtained CS nano-sized microcapsules were characterized with the infrared spectra（IR）, scanning electron microscope （SEM） and the particle size analyzer. The model protein, bovine serum albumin（BSA） as a model drug, was encapsulated into the CS/TPP nano-sized microcapsules. Infrared spectrum of CS/TPP nano-sized microcapsules indicated that CS was cross-linked with TPP. The average di- ameter of the obtained nano-sized microcapsules was around 430 nm, and a homogeneous size distribution and good dispersion were observed. BSA-loading efficiency of CS/TPP nano-sized mierocapsules did not increase with the increase in concentration of CS. When mass fraction of CS was 0.25% , the highest BSA-loading efficiency of CS/TPP nano-sized microcapsules reached 79.74%, while BSA continually and smoothly released up to one week.