摘要
以毛毡杜鹃嫩茎段为外植体,应用均匀设计法筛选最适合的培养基。试验结果表明,适合嫩茎段腋芽萌发生长及生根的最佳培养基为:MS(改良)+IAA 0.10 mg/L+IBA 0.30mg/L+NAA 0.12mg/L+GA31.80mg/L,再生率达93%以上。以再生植株的茎节为材料进行快繁的结果表明,在25天的一个培养周期内,茎段的增殖倍数平均达50以上。
Take young stems of Rhododendron confertissimum as explants and suitable medium compositions for axillary bud growing and rooting were screened through uniform design experiments. The results showed that MS （modified） ＋ IAA 0. 10 mg/L ＋ IBA 0. 30 mg/L ＋ NAA 0. 12 mg/L ＋ GA 31. 80 mg/L was the most suitable with a regeneration rate of 93%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 50 -fold proliferation rate of stems was achieved within 25 days.
出处
《林业科技》
2009年第2期57-59,共3页
Forestry Science & Technology
基金
吉林省科技厅资助项目(200705C05)
通化师范学院自然科学基金项目(XS070076)
关键词
毛毡杜鹃
组织培养
快繁
均匀设计
Rhododendron confertissimum
Tissue culture
Micropropagation
Uniform design
