乳腺良恶性病变中Survivin Cyclin D1和Bcl-2蛋白的表达及意义

The Protein Expression and Its Significance of Surivin,CyclinD1 and Bcl-2 in the Different Breast Lesions

目的:探讨乳腺导管普通型增生(UDP)、不典型增生(HDP)、癌(Ca)病变发展过程中,Survivin、Cyclin D1和Bcl-2蛋白表达及意义。方法:应用免疫组织化学技术S-P法检测Survivin、Cyclin D1和Bcl-2蛋白在30例UDP、30例HDP、40例癌(Ca)病变中的表达。统计学方法采用卡方检验分析比较各组病变中Survivin、Cyclin D1和Bcl-2蛋白表达的差别及其相关性。结果:①Survivin在UDP、HDP、Ca中的阳性表达率分别为16.67%(5/30)、26.67%(8/30)和95.00%(38/40),Ca与UDP、HDP之间阳性表达率具有显著性差异(P<0.01)。表达范围和表达强度随UDP到HDP到Ca呈逐渐递增的趋势。②Cyclin D1在UDP、HDP、Ca中的阳性表达率分别为23.33%(7/30)、40.00%(12/30)和65.00%(26/40),其中,UDP与Ca之间具有显著性差异(P<0.01),其表达范围和表达强度随UDP到HDP再到Ca也呈逐渐递增的趋势。③Bcl-2在乳腺UDP、HDP、Ca中的阳性表达率分别为83.33%(25/30)、73.33%(22/30)和67.50%(27/40);虽表达率逐渐降低,但组间比较三者之间均无统计学意义(P>0.05)。表达范围和着色强度随UDP到HDP再到Ca呈逐渐递减的趋势。结论:Survivin和Cyclin D1蛋白在乳腺Ca发生的多步骤、多阶段过程中发挥促进作用,Bcl-2蛋白的表达在乳腺组织恶性转化进程中可能起负性调节作用。

Objective： In order to probe the possible role of Cyclin D1, Surivin and Bcl - 2 in carcinogenetic process from usual ductal hyperplasia （UDP） , atypical dutcal hyperplasia （HI）P） to carcinoma in situ and infiltrating carcinoma. Method： The protein expression of the Cyclin D1, Survivin and Bcl- 2 in 30 cases of UDP, 30 cases of HDP and 40 cases of carcinoma in situ and infiltrating carcinoma was detected respectively by immunohis- - tochemistry （S- P）. The positive expression rates among these lesions were analyzed by statistics. Result： ①The positive expression rates of survivin in UDP, HDP and breast cancer were respectively 16.67 % （5/30）, 26.67 % （8/30）, and 95.00 % （38/40）, with a significant difference between UDP and cancer （P 〈 0.01） and between HDP and cancer （P〈0.01）. Also, the extent and intensity of positive expression were increased from UDP, HDP to cancer . ②The positive rates of Cyclin D1 expression in UDP, HDP and breast cancer were respectively 23.33% （7/30）, 40.00% （12/30） and 65.00% （26/40） with a significant difference between UDP and cancer （P 〈 0.01 ） in a similar pattern with Survivin for extent and in- - tensity of expression. ③ The positive rates of Bcl - 2 expression were respectively 83.33 % （25/30）, 73.33 96 （22/30） and 67.50（27/40） in these three groups. There was no significant difference among these groups. But, the extent and intensity of positive expression were decreased from UDP, HDP to cancer. Conclusion： Survivin and CyclinD1 play important roles in promoting malignant transforma- - tion, while Blc- 2 may have a negative effect on the malignant transformation.