线粒体融合素-2对裸鼠成瘤性及增殖、转移的影响

The effect of mitofusin-2 gene on the neoplasia, proliferation and metastasis of human breast carcinoma cells in vivo

目的 观察线粒体融合素（mfn）-2对裸鼠成瘤性及增殖瘤的增殖、转移的影响。方法裸鼠共分为3组：实验组、空载体对照组、空白对照组，分别将3组MCF-7细胞异种移植到无胸腺小鼠体内，建立裸鼠人乳腺癌移植瘤模型，比较3组裸鼠之间肿瘤的大小和重量，逆转录-聚合酶链反应（RT—PCR）检测3组肿瘤组织mfn2的表达，免疫组织化学半定量检测核增殖抗原（Ki-67）、血管内皮生长因子（VEGF）的表达。结果实验组、空载体对照组、空白对照组3组肿瘤瘤体质量（单位：g）分别为1．78±0．13、2．84±0．16、2．77±0．12，实验组裸鼠肿瘤重量明显低于对照组（P〈0．05），实验组瘤体的体积与两对照组比较差异有统计学意义（P〈0．05），实验组mfn2基因高表达（P〈0．05），两对照组低表达，两对照组之间差异无统计学意义（P〉0．05），实验组与两对照组比较，实验组Ki-67的表达升高，而VEGF的表达明显降低（P〈0．05）。结论mfn2基因可明显抑制人乳腺癌成瘤，对人乳腺癌裸鼠移植成瘤后的增殖和转移能力有影响。

Objective To investigate the effects of Mitofusin-2 gene （mfn2） on the neoplasia, proliferation and metastasis of Human breast carcinoma cells in vivo. Methods The cells were divided into three groups：non-transfection group as negative control group, pEGFP-C2 transfection group as experimental control group, and pEGFPmfn2 transfection group as experimental group. Fifteen nude mice were randomly divided into 3 groups：the experimental group, the experimental control group, and the negative groups that were heterografted with three groups＇ cells respectively. The expression of mfn2 was detected by RT-PCR in tumor tissues. The expression of Ki-67 and VEGF was detected by immunohistochemistry. Results The tumor weight in negative control, experimental control and experimental groups was （2.77 ± 0. 12）, （2.84 ±0. 16） and （1.78± 0. 13 ） g, respectively. The tumor weight in experimental group was significantly lighter than in two control groups （ P 〈 0.05 ）. The tumor volume in experimental group was markedly less than in experimental control and negative control groups （P 〈 0.05 ）. The expression of mfn2 in experimental group was higher than in experimental control and negative control groups （P 〈 0.05 ）. The expression of Ki-67 in experimental group was higher than in experimental control and negative control groups （P 〈 0.05）, but the expression of VEGF in experimental group was lower than in experimental control and negative control groups （ P 〈 0.05 ）. Conclusion Exogenous mfn2 can strongly inhibit proliferation and metastatic progression of breast carcinoma MCF-7 in xenograft implantation model.