血清胰岛素样生长因子-Ⅱ、血管内皮生长因子、白细胞介素-Ⅱ和干扰素-γ水平在肝癌微波消融治疗前后的变化

The changes of sera IGF- Ⅱand VEGF concentrations before and after microwave ablation treatment in rat models of hepatocellular carcinoma

目的观察大鼠在肝癌微波消融治疗前后血清胰岛素样生长因子（IGF）-Ⅱ、血管内皮生长因子（VEGF）、白细胞介素（IL）-2和干扰素（IFN）-γ水平的变化。方法80只SD大鼠，随机分4组，正常组除抽血检测外不做任何处理，其余3组行肝癌细胞种植，对照组尾动脉抽血检测，手术组行手术切除肿瘤，消融组消融肿瘤，ELISA法测定血清IGF—Ⅱ、VEGF、IL-2和IFN-γ／水平并比较3周后4组间IGF—Ⅱ、VEGF、IL-2和IFN-γ水平的变化情况。结果对照组第5周较第3周时血清IGF—Ⅱ[（1．93±0．45）μg／L比（1．16±0．21）μg／L]和VEGF[（395．72±55．28）ng／L比（248．43±41．71）ng／L，P〈0．05]。经手术治疗或微波消融治疗后血清IGF-Ⅱ（0．65±0．18比0．73±0．16）和VEGF[（129．35±51．46）ng／L比（135．63±33．37）ng／L]水平明显下降，而IL-2、IFN-γ水平则明显升高。结论IGF—Ⅱ可直接或通过增加VEGF的合成促进肿瘤血管形成，可作为原枯忡肝癌的诊断、判断愈后与跟踪随访的重要指标。微波消融治疗肝癌是一种有效的方法。

Objective To study change of IGF- Ⅱ , VEGF, IL-2 and IFN-γ before and after microwave ablation in rat models of hepatocellular carcinoma （HCC）. Methods Eighty SD rats were randomly divided into 4 groups as follows ： normal group, control group, microwave ablation group and surgical operation group. The HCC models were established in control group, microwave ablation group and surgical operation group. The microwave ablation group and surgical operation group accepted microwave ablation and surgical operation respectively. The serum concentrations of IGF-Ⅱ , VEGF, IL-2 and IFN-γ were determined by ELISA. IGF-Ⅱ, VEGF, IL-2, AFP and IFN-γ concentrations between the normal and control groups were compared after treatment for 3 weeks. Results In control group, sera concentrations of IGF- Ⅱ（ 1.93± 0.45 vs 1.16 ± 0. 21 ）μg/L, and VEGF （395.72 ± 55.28 vs 248.43 ±41.71 ） ng/L on the 5th week were significantly higher than those on the 3rd week （ P 〈 0.05 ）. After microwave ablation or surgical operation, concentrations of IGF- Ⅱ （0.65 ±0.18 vs 0. 73 ±0.16） μg/L and VEGF （ 129.35 ±51.46 vs 135.63 ± 33.37） ng/L were significantly decreased,but IL-2, and IFN-γ concentrations significantly increased as compared with control group. Conclusion IGF- Ⅱ can promote tumor angiogenesis directly or by increasing the synthesis of VEGF. It could be an important index in diagnosis, prognosis and tracking of primary HCC. Microwave ablation is an effective method in the treatment of HCC.