青蒿素作用前后K562细胞基因表达谱的改变

An alteration in the gene expression profiling of K562 cells after treatment with artemisinin

目的:利用基因表达谱芯片研究青蒿素对体外培养的人红白血病K562细胞基因表达谱的影响。方法:青蒿素处理K562细胞24h后,分别提取处理组和对照组细胞的总RNA,将两组RNA纯化为mRNA,逆转录成cDNA,用Cy3和Cy5两种不同的荧光染料进行线性扩增标记,与人全基因组基因表达谱芯片杂交,采用生物信息学方法分析青蒿素处理前后K562细胞基因表达谱的改变。结果:总共发现差异基因238个,其中上调基因136个,下调基因102个,并对其进行生物学功能分类。结论:应用全基因组表达谱芯片并结合Panther生物学软件分析差异表达基因,青蒿素作用K562细胞主要是通过细胞毒作用诱导细胞凋亡,而抑制细胞生长作用并不明显。

Objective To investigate the effect of artemisinin on the gene expression profile of human erythroleukemia K562 cells cultured in vitro. Methods The total RNAs were extracted from K562 cells treated with ART for 24 hours and from the control K562 cells. All the RNAs were purified into mRNA and then converted to double-stranded cDNAs by reverse transcription. The cDNAs were labeled with a fluorescence dye Cy3 or Cy5 and hybridized to a human whole-genome oligonucleotide microarray. The change in the gene expression profiling of K563 cells following treatment with artemisinin was analyzed by using bioinformatics. Results A total of 238 differential genes were found, 136 of which were up-regulated and 102 were down-regulated. Their biologic function was classified. Conclusions By analyzing the differential gene expressions with the whole-genome oligonucleotide microarray combined with the Panther software, we find that artemisinin induces the apoptosis of K562 cells by its cytotoxic effect on the cells and that the inhibitory effect of artemisinin on the cell growth is not evident.