肺组织瓣内衬壳聚糖管用于修补食管缺损的实验研究

Pulmonary tissue flap with inner chitosan tube stent repairing esophagus defection

目的:探讨肺组织瓣内衬壳聚糖管修补食管缺损的可行性。方法:取15只日本大耳白兔,分为两组,实验组10只,行自体肺组织瓣修补中段食管部分缺损(内衬壳聚糖管支架);对照组5只,无内衬支架。术后第2、4、8周时,大体及组织学观察缺损修补处肺组织瓣情况,术后10周对存活兔钡餐透视观察食管通畅情况。结果:10只实验动物存活超过2周以上,肺组织瓣与食管缺损处牢固愈合,肺组织瓣表面有鳞状上皮化生。术后10周,食管钡餐检查见实验组食管钡剂通过顺利,无明显狭窄及反流,蠕动尚可,对照组轻度狭窄,无明显梗阻及反流。结论:肺组织瓣修补食管缺损是一种可行的方法,壳聚糖管可以作为内衬支架防止狭窄。

Objective To explore the feasibility of pulmonary tissue flap with inner chitosan tube stent repairing esophagus defection. Method 15 Japanese big ear white rabbits were assigned to receive pulmonary tissue flap repairing center-section esophagus partial defection, in which 10 with inner chitosan tube stent （experiment group） and 5 with no inner chitosan tube stent （control group）. The status of pulmonary tissue flap was observed roughly and histologically 2, 4, and 8 weeks after surgery, esophagus unobstructed situation was observed by barium meal examination 10 weeks after surgery. Results 10 rabbits survived longer than 2 weeks, the junction of pulmonary tissue flap and esophagus became scarred, the surface of pulmonary tissue flap was covered by squamous metaplasia. There was no obvious stenosis or back flow in experiment group and there was mild stenosis, no obvious obstruction or back flow in control group 10 weeks after surgery. Conclusions It is a feasible method using pulmonary tissue flap to repair esophagus defection, Chitosan tube stent as inner support can prevent esophagus stenosis.