系统性红斑狼疮患者基因组DNA甲基化水平的研究

A study of genomic DNA methylation in patients with systemic lupus erythematosus

目的:检测系统性红斑狼疮(SLE)患者基因组DNA甲基化的水平,探讨其在SLE发病中的意义。方法:抽提SLE患者和正常人DNA,采用高效毛细管电泳法检测基因组DNA甲基化水平。结果:SLE组DNA甲基化水平低于正常对照组,差异有统计学意义(P<0.05),SLE活动组及SLE稳定组与正常对照组之间差异均有统计学意义(P=0.012,P=0.008),而SLE活动组与稳定组之间甲基化水平差异无统计学意义(P=0.952);SLE患者DNA甲基化水平与系统性红斑狼疮疾病活动指数、抗核小体抗体、抗C1q抗体、抗核抗体、抗dsDNA抗体、免疫球蛋白、补体C3、C4无相关性。结论:SLE患者基因组DNA甲基化水平降低。

Objective To detect the methylation levels of genomic DNA in patients with systemic lupus erythematosus （SLE）. Methods DNA was extracted from SLE patients and healthy controls. DNA methylation levels were detected by high-performance capillary electrophoresis. Results The levels of DNA methylation was lower in the SLE patients than in the controls （P 〈 0.05 ）. DNA methylation levels differed significantly between the patients with active SLE or with stable SLE and the controls （P = 0.012 and P = 0.008, respectively）, but no significant difference was found between active SLE and stable SLE. DNA methylation levels were not associated with SLE disease activity index （SLEDAI）, anti-nucleosome antibodies, anti-C1q antibodies, ANA, anti-dsDNA antibodies, immunoglobulin, and complements C3 and C4. Conclusions The methylation levels of genomic DNA are decreased in patients with SLE.