重度子痫前期胎盘组织中差异表达蛋白研究

Study on proteins from placenta tissue expressed differently in patients with preeclampsia

目的寻求与重度子痫前期发生相关的蛋白,为重度子痫前期发病机制研究奠定基础。方法选择2008年1～3月在天津医科大学总医院产科住院分娩的重度子痫前期患者及正常妊娠妇女各8例,采集正常孕妇和重度子痫前期患者的胎盘组织,提取总蛋白后进行双向电泳技术进行分离。蛋白质凝胶图像经PDQuest软件分析后,获得差异蛋白质点。对差异蛋白质点进行时间飞行质谱(MALDI-TOF-MS)鉴定。结果与正常妊娠相比,重度子痫前期患者胎盘组织丰度差异表达相差2倍以上,并经Student's t-test检验差异有统计学意义的蛋白质点有14个(P<0.05),其中9个点在重度子痫前期组表达上调,另5个点表达下调。这些蛋白广泛参与细胞信号转导、细胞凋亡、应激反应、细胞结构、能量代谢等生理过程。结论重度子痫前期的发生中,胎盘组织可能存在独特的蛋白表达模式。

Objective For further study on the pathological mechanism, related proteins from placenta tissue were sought in patients with preeclampsia （PE）. Methods Total proteins extracted from placenta of normal and PE patients were electrophoresed using two-dimensional gel （2-DE） method and gel images were compared by PD Quest ver7.4 image analysis software. Difference spots were isolated and identified using matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）. Results Compared with the normal placenta, 14 protein spots with 2 fold of difference statistically in PE placenta were found including nine up-regulated and five down-regulated protein spots. Af- ter identification, proteins of the 14 spots involved in the physiological process of signal transduction, cell apoptosis, stress reaction, cell construction and energy metabolism. Conclusion A specific protein expression model may exist in placenta of PE.