关木通对大鼠的致肿瘤作用及丹参的干预

Effect of Salvia Miltiorrhiza on the Aristolochic Acid-Induced Tumors in Rats

目的:观察丹参对马兜铃酸诱导的大鼠胃及膀胱肿瘤病变的影响。方法:给大鼠灌服关木通浸膏(含马兜铃酸10mg·kg-1.d-1)12～24周,并给予不同剂量丹参水溶液(5、10、15g·kg-1.d-1),于实验第12、16、20、24周处死大鼠,留取胃、膀胱及肾脏标本,观察其病理学改变。结果:实验第12周开始,所有马兜铃酸诱导的大鼠前胃均出现不同程度的病理改变,胃黏膜可见乳头状增生或乳头状瘤,并伴有上皮轻-重度异型增生,以模型组病变明显,并随实验进展而程度加重、范围扩大,出现原位癌病变。模型组大鼠膀胱黏膜内可见乳头状增生或乳头状瘤,至实验第24周,病变加重,可见多发性乳头状瘤伴局灶上皮异型增生,并有淋巴细胞浸润,部分大鼠膀胱组织表现为高分化原位癌。丹参干预后胃及膀胱病变有所减轻。马兜铃酸诱导的大鼠肾组织主要表现为肾小管间质病变,未出现癌变表现。结论:丹参干预后可减轻马兜铃酸诱导的大鼠胃组织及膀胱病变,具有一定的抗肿瘤作用。

Objective：To investigate the effect of Salvia miltiorrhiza（SM） on the aristolochic acid- induced tumors in rats. Methods：Wister rats were given the extracta of Caulis Aristolochiae Manshuriensis, and then administrated SM 5,10,15 g·kg^-1·d^-1 for 12 to 24 weeks. All rats were sacrified at the end of the 12th, 16th,20th,24th week. The pathological changes of stomach, bladder and kidney were observed through the staining of HE. Results： There appeared papillary hyperplasia or papilloma accompanied with epithelial dysplasia and even carcinoma in situ in gastric mucosa in aristolochic acid rats. It Can be seen Multiple papilloma accompanied with epithelial dysplasia and Lymphocyte infiltration in Bladder mucosa in model rats. The pathological changes of stomach and bladder Mitigated After SM intervention. The renal pathological change lccated in tubulars and interstitial, such as disappearance of brush border, cells degeneration and mild fibrosis along with the experiment progress. There was no cancer performance in kidney. Conclusion： SM could ameliorate the pathological changes of stomach and bladder in aristolochic acidinduced rats and it may have the anti - tumor effects.