PCR-DGGE技术及其在植物微生态研究中的应用

Application of PCR-DGGE Technology in the Research of Micro-ecology of Plants

植物中微生物种类较为丰富,在植物微生态系统中发挥重要作用。变性梯度凝胶电泳(denaturinggradient gel electrophoresis,DGGE)技术作为研究微生物物种多样性和种群动态变化的分子检测工具之一,广泛应用于植物微生物群落多样性和群落动态变化研究中。概述了DGGE技术的原理,及其在植物微生物生态学中的应用以及在该领域研究中导致DGGE偏差的因素和相应解决方法,对DGGE检测结果的分析提供参考,为进一步研究植物微生物相互作用提供帮助。

The microorganism that is abundant in plant,plays importane roles in plant micro-ecology. As a fast and reliable molecular method,polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） technique has been proven to be a powerful tool for analyzing microbial diversity and microbial communities and their dynamics changes in plant micro-ecology. In this paper,the principle and application aspects in plant micro-ecology,as well as main factors that can limit DGGE techniques for microbial ecology analysis were introduced. It is expected to provide basis for the analysis of the DGGE result and in the plant-microbial interaction research.