猪下丘脑发育差异表达基因的分离、克隆与表达

Cloning and Characterization of the Difference of Gene Expressed in Pig Hypothalamus

在小梅山猪下丘脑不同发育阶段,用mRNA差异显示技术分离并筛选出一个差异表达片段,命名为CQ01,221 bp。测序与BLAST分析发现,CQ01与猪血管内皮生长因子A(VEGFA)高度同源96%。半定量分析结果显示,CQ01在初情期时下丘脑中表达量较高。

One specific fragment which named CQ01 （221 bp） was found from hypothalamus of Meishan pig by differential display reverse transcriptase polymerase chain reaction （DDRT-PCR）. After secquencing and BLAST analysis, CQ01 displayed high homology （96%） to VEGFA gene of pig. The result of semi-quantitative showed that the express of CQ01 was high at puberty.