溶解肠杆菌的交流电电解刺激过程

Electrolytic Stimulation of Enterobacter dissolvens Cultivated in Glucose Medium

以葡萄糖为惟一碳源,研究了溶解肠杆菌(Enterobacter dissolvens)在外加电场交流电的刺激下细胞的生长和代谢的过程。研究表明,在低电流10 mA刺激下,交流电刺激下的细菌没有明显的刺激效应;当采用100 mA的电流通电24 h,交流电刺激下的细菌出现了明显的刺激效应,当通电12 h后,菌液葡萄糖的降解率和脱氢酶活性分别为对照参比的1.4倍和2.17倍,细胞浓度呈现明显的增长。分析原因可能是由于电极产物中没有过氧化氢的产生。

Behavior of E. dissolvens under the exposure to weak alternating current （AC） was investigated by using glucose as sole carbon source. When 10 rnA current were applied,water electrolysis was not induced in the process of AC stimulate,while the use of 100 mA led to obvious increases of cell growth and glucose uptake. After 12 hour electrolytic stimulation,the specific dehydrogenase activity and glucose consumption was 1.4 and 2.17 fold respectively,as much as that of the controls. The enhanced cell growth could be seen as a result of absence of peroxide under the application of AC.