摘要
DArT是一种依赖于基因芯片杂交基础之上的分子标记技术,由于其具有省时、高通量、低花费、无需预知研究对象的DNA序列等优点而被应用到植物遗传研究中。文章介绍了DArT技术的原理、特点、具体操作方法及其目前在植物遗传研究中的应用。
Diversity arrays technology (DART) is a microarray hybridization-based molecular marker technique, and a novel method to discover genetic polymorphic markers. DArT has recently been applied in genetic research of plants because of its characteristics of sequence-independent, high-throughput, fast, low-cost. We briefly introduce the principle, characteristics, protocol of DArT and its application in genetic research of plants.
出处
《遗传》
CAS
CSCD
北大核心
2009年第4期359-364,共6页
Hereditas(Beijing)
基金
江苏省自然科学基金项目(编号:BK2007080)资助
关键词
分子标记
DArT技术
杂交
molecular markers
diversity arrays technology
hybridization