凝血酶原基因G20210A单核苷酸多态性与人工关节置换术后深静脉血栓的相关性

Correlation between single nucleotide polymorphism of prothrombin gene G20210 and deep vein thrombosis after total joint replacement

目的检测凝血酶原基因G20210A单核苷酸多态性在人工关节置换术患者中的分布频率，从而评价其与人工关节置换术后发生深静脉血栓形成（DVT）的相关性。方法采用聚合酶链反应（PCR）及DNA直接测序的方法分析其中55例患者的凝血酶原基因G20210A突变发生率以及该突变与人工关节置换术后DVT形成的相关性。结果55例患者中术后下肢深静脉造影显示27例有DVT，28例无DVT；凝血酶原基因G20210A突变发生率为0，DVT患者与无DVT患者凝血酶原G20210A基因型变异频率均为0，两组突变基因的频率也均为0。两组间比较差异无统计学意义（X^2=0，P〉0．05）。结论中国人凝血酶原基因G20210A突变罕见，与人工关节置换术后DVT的发生无相关性；首次得出中国人人工关节置换术后DVT发生的易感人群中血浆凝血酶原活性升高与凝血酶原基因G20210A突变无关，可能存在另外的凝血酶原基因多态性或其他发病机制。

Objective To evaluate the correlation between prothrombin gene G20210A mutation and deep vein thrombosis after total joint replacement through detecting distribution frequency of single nucleotide polymorphism of the gene in patients undergoing total joint replacement. Method PCR and direct sequencing of DNA are used to analyze the frequency of prothrombin gene G20210A mutation in 55 patients undergoing total joint replacement, and the relationship between the mutation and deep vein thrombosis after total joint replacement is evaluated. Result Morbidity of prothrombin gene G20210A mutation in 55 patients is O, both in either DVT group （27 patients ） or non-DVT group （28 patients ）. There is no significant differentia between the two groups （ P 〉 0. 05 ）. Conclusion Mutation of prothrombin gene G20210A in Chinese patients is rare, and there is no correlation between the genetic mutation and deep vein thrombosis after total joint replacement.