免疫佐剂对幽门螺杆菌免疫原性的影响

Effect of different immune adjuvants on the immunogenicity of Helicobacter pylori in mice

幽门螺杆菌是一类高传染性的致病细菌,能导致人类多种疾病。为比较不同佐剂对该致病菌的免疫原性的影响,实验中利用简单的布氏肉汤添加环糊精液体培养基体外培养幽门螺杆菌,分别与福氏佐剂、自制油佐剂、氢氧化铝佐剂混合免疫昆明种小鼠,经间接ELISA法分析抗血清效价证实,三种免疫佐剂都能有效地刺激小鼠对幽门螺杆菌产生明显的体液应答,其中福氏佐剂的效果最好,自制油佐剂略强于氢氧化铝佐剂的免疫活化作用。三者免疫的抗血清效价分别为,福氏佐剂1∶25600,自制油佐剂1∶12800,氢氧化铝佐剂1∶12800。

Helicobacter pylori （Hp） is an extensively epidemical bacterium, which leads to many human diseases. In order to evaluate different adjuvants effect on the immanogenicity of Helieobacter pylori in mice, three adjuvants including Freund＇s, self-made oil and aluminum hydroxide adjuvants were mixed respectively with Hp J99 cultivated in Brucella Broth medium adding 10% 13-cyclodextrin and were used to immunize Kunming mice. After immunization three times, mice antiserum titre was detected with indirect enzyme-linked immunosorbant assay （ ELISA）. The result showed that Freund＇ s adjuvant is of the strongest stimulation effect in mouse immunization than those of two other adjuvants and that positive serum titre is 1：25600 with Freund＇ s adjuvant, 1：12800 with self-made oil adjuvant and 1：12800 with aluminum adjuvant respectively. Otherwise, the costs of self-made oil and aluminum adjuvants are much lower than that of Freund＇ s adjuvant and are adapt to being extensively applied in industrial immune preparation.