摘要
为了探讨抗-HCV检测设置灰区的意义。以阈值定为阴性对照加0.12,灰区定为阈值下20%的范围,落在灰区内的样本用同一试剂双孔复查,只要有一孔阳性则判为阳性。结果显示,27份样本中,经复查有4份阳性,占14.8%,13份仍落在灰区,占48.1%,10份为阴性,占37%。如果不设灰区,将有抗-HCV阳性的样本的血液流入到患者体内,可危及患者的身体健康。
To go into significance of setting gray areas in the process of anti-HCV detetion. The antibodies against HCV of 27 samples were detected by ELISA, the negative control value plus 0.12 was determined as threshold, and the scope of 20 percent under the threshold was designated by the name of gray area. Using the same reagents , the specimen in which its tested value belong to within the gray area was retested through two holes, as long as three is one of holes-positive, the result was determined into the positive. The results showed that 4 of 27 samples （ 14.8% ） , exhibit positive; 13 samples （48.1%） whose values belong to within the gray area, the results of 10 samples 137% ） exhibit negative. Therefore, it is necessary to set the gray area in the process of anti-HCV detection in order to ensure the safety of transfusion.
出处
《微生物学免疫学进展》
2009年第1期39-40,共2页
Progress In Microbiology and Immunology
关键词
灰区
抗-HCV
意义
Gray areas
Anti- HCV
Significance
