脐带血采集中微生物污染原因分析

Cause analysis of microbiological contamination in cord blood collection

目的探讨脐带血污染的检测周期并分析采分过程中脐带血微生物污染的影响因素。方法采用3D240全自动细菌培养系统对1000份脐带血进行采集后和分离后28d的培养并对阳性瓶镜检。结果1000份脐带血中,31份培养阳性全部在10d内报警,阳性率为3.1%,其中30份采集污染,1份分离污染;自然分娩和脐血量50g以下阳性率明显高于总体阳性率。结论培养周期不应小于10d,采集过程是导致污染的重要原因,污染率与生产方式、采集量等相关。

Objective To investigate the test period of the umbilical cord blood contamination and analyze the influencing factors of the microbiological contamination of the cord blood during the collection and separation process. Methods 1 000 samples of the umbilical cord blood were collected and separated by using the 3D240 fullautomatic cell culture system, and the positive samples were examined under microscope after 28-day culture. Results Among 1 000 samples, 31 positive samples, of which 30 samples were contaminated during collection and 1 contaminated during the separation, were all alert in 10 days; the positive rate is 3.1 percent. The positive rate of samples for natural labor or those for cord blood weight less than 50 grams is obviously higher than the overall positive rate. Conclusion The culture period cannot be less than 10 days. The cord blood is mainly contaminated during the collection period. The contamination rate is＇dependent on the delivery style and the cord blood weight collected. Key words cord blood contamination rate collection separation natural labor abdominal delivery third stage of labor