摘要
目的研究人食管鳞状细胞癌细胞金属硫蛋白-3(MT-3)mRNA的表达。方法选取5种人食管癌细胞株,其中TE-1、TE-13、TTN和ECA-109为食管鳞状细胞癌细胞株,OE33为食管腺癌细胞株。应用RT-PCR技术检测各细胞株中MT-3 mRNA的表达。以正常人外周血单核细胞作为正常对照。结果RT-PCR产物回收经DNA序列测定表明为目的基因MT-3 mRNA。凝胶电泳图像显示所有被测样本均有MT-3 mRNA表达;数据分析显示,人食管癌细胞株TE-1、TE-13、TTN、ECA-109和OE33中MT-3 mRNA的相对表达量分别为0.230±0.023、0.516±0.020、0.140±0.009、0.176±0.015和0.085±0.011,明显低于正常对照的0.762±0.026,经比较差异均有统计学意义(P<0.01)。结论人食管鳞状细胞癌细胞株中广泛存在MT-3 mRNA表达水平下降。提示食管鳞状细胞癌的发生可能与MT-3 mRNA的异常低表达有关。
Objective To detect the expression of metallothionein-3 （MT-3）mRNA in human esophageal squamous cell carcinoma. Methods Five cell lines of human esophageal cancer,TE-1,TE-13,TTN,ECA-109 （cell lines of esophageal squamous cell carcinoma） and OE33 （cell lines of esophageal adenocarcinoma）,were used in this study. RT-PCR was employed to detect the expression of MT-3 mRNA. Peripheral blood monouclear cells from normal subjects were served as controls. Results Sequencing of RT-PCR product certified the gene of MT-3 mRNA. It was revealed by gel electrophoresis that there was expression of MT-3 mRNA in each cell line. The relative expression of MT-3 mRNA was 0.230±0.023,0.516±0.020,0.140±0.009,0.176±0.015 and 0.085±0.011 in cell lines of TE-1,TE-13,TTN,ECA-109 and OE33,respectively,significantly lower than that in controls （0.762±0.026） （P〈0.01）. Conclusion There are decrease of MT-3 mRNA expression in the cell lines of human esophageal squamous cell carcinoma,indicating the carcinogenesis of esophageal squamous cell carcinoma may be related to the abnormally low expression of MT-3 mRNA.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2009年第3期319-322,共4页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(30400527)
河北省科学技术研究与发展计划项目(08276101D-9)~~
