藕节促凝血有效组分的筛选及凝血作用研究

Study on the Screening and Coagulant Activity of the Effective Procoagulant Components in Nodus nelumbinis Rhizomatis

筛选藕节促凝血的有效组分。采用毛细管法测定小鼠凝血时间(CT);采用剪尾法测定小鼠出血时间(BT);采用试管法测定新西兰兔活化部分凝血活酶时间(APTT),凝血酶原时间(PT)和凝血酶时间(TT)。结果表明,藕节提取物组分Ⅰ和组分Ⅱ均表现出较好的促凝血作用,组分Ⅲ没有活性。说明藕节促凝血的有效组分为组分Ⅰ和组分Ⅱ。

The present study was to screen the effective procoagulant components from Nodus nelumbinis Rhizomatis. Coagulation time（CT）was tested by capillary method in mice. Bleeding time（BT） was determined by cutting-tail method in mice. Activated partial thromboplastin time （APTT）, prothrombin time（PT）and thrombin time （TT）were evaluated by test tube method in rabbit. The results suggest that component Ⅰ and component Ⅱ of nodus nelumbinis rhizomatis exhibit procoagulant function,and component Ⅲ does not have the activity of procoagulant. The results indicate the effective procoagulant components in nodus nelumbinis rhizomatis are component Ⅰ and component Ⅱ.