油菜籽粒发育过程中PEPCase活性与油脂、蛋白及亚基积累的特点

The characteristics of PEPCase activity and accumulation of oil,protein and major protein subunits during seed development of rape(Brassica napus)

以油菜品种秦优7号为材料,研究籽粒发育过程中PEPCase活性变化规律以及水份、油脂、蛋白、主要贮藏蛋白亚基等含量的积累动态,以及它们之间的相关性。结果表明,种子发育期间,水份含量从81.61%下降到23.22%,油脂含量从0.26%上升到31.38%,蛋白含量从1.1%上升到21.36%,可溶性蛋白含量从0.63%上升到12.21%。12S球蛋白先积累,2S清蛋白后积累;PEPCase活性与蛋白含量、油脂含量分别呈阶段性相关,与油脂含量/蛋白含量比值呈极显著负相关;与12S组分含量呈显著负相关,与α2-3,α4,β2,β3,β4亚基含量呈显著负相关,与其它亚基含量的相关性不显著;与2S组分及其亚基含量相关性不显著。

The dynamic accumulation of moisture, oil, total protein, major storage protein （2S and 12S fractions） and their subunits, and variation of PEPCase activity during seed development of rape were investigated in this study with the variety of Qinyou No7. The correlations between PEPCase activity and these seed components were further studied. The results showed that during seed development, moisture content decreased from 81.61% to 23.22%. The content of oil,protein and soluble protein increased from 0.26% to 31.38% , 1.1% to 21.36% and 0.63% to 12.21% respectively. The accumulation of 12S started earlier than that of 2S. The correlations between the activity of PEPCase and the protein content, and oil content were found during different stages of seed development; negative correlations were found between the activity of PEPCase and oil content/protein content ratio, as well as the content of 12S α2-3,α4,β2,β3,and β4 ;No correlation was found between the activity of PEPCase and 2S fraction content and its subunits.