芝麻黄花叶病病原的分子鉴定

Molecular identification of peanut stripe virus isolated from Sesame(Sesamun indicium L.)

从芝麻黄花叶病株上分离得到花生条纹病毒2个分离物（Peanut stripe virus sesame isolate，PStV-sel和PStV-se2）。提取感病芝麻叶片的总RNA，RT-PCR扩增外壳蛋白基因（cp）片段。序列测定结果显示，印基因含有861个核苷酸，编码分子量为32．4kDa的蛋白。2个株系cp基因与PStV其它株系核苷酸序列一致性为94．4％～99．9％，氨基酸序列一致性为93．7％～100％。株系间CP氨基酸序列系统进化树结果表明，芝麻PStV分离物与中国其它寄主来源的PStV株系处于同一进化分支。

The coat protein gene （cp） of peanut stripe virus -sesame isolates （PStV- se） was amplified by RT -PCR and sequenced. Coat protein gene was 861 nt in length, identity of its nucleotide sequence with those other PStV strains was 94.4 - 99.9% ,identity of amino acid sequence was 93.7 - 100%. Phylogenetic analysis of sequence of the coat protein showed that PStV - se was closely related to the previously - characterized PStV strains from China rather than with those PStV strains from Southeast Asia.