植物雌激素对体外培养的大鼠睾丸间质细胞分泌睾酮的作用

Effects of Phytoestrogens on Testosterone Production of Rat Leydig Cells

目的:研究植物雌激素(大豆苷元、染料木素)对睾丸间质细胞分泌睾酮的刺激作用,并初步研究其可能的分子作用机制。方法:采用Percoll不连续密度梯度离心,分离获得3月龄Sprague-Dawley(SD)大鼠的睾丸间质细胞。以人绒毛膜促性腺激素(hCG)为阳性对照药物,测定不同浓度(0、0.02、0.1、0.5、1、5μmol/L)大豆苷元、染料木素对睾丸间质细胞分泌睾酮的影响。RT-PCR检测睾酮生物合成过程中胆固醇侧链裂解酶细胞色素P450(P450scc)的表达。结果:0.1μmol/L的染料木素能显著刺激原代大鼠睾丸间质细胞的睾酮分泌,RT-PCR结果显示染料木素能显著上调睾酮合成过程中P450sccmRNA的表达。在较高浓度下(5μmol/L),大豆苷元和染料木素均能抑制睾丸间质细胞分泌睾酮。结论:染料木素在低浓度下(0.1μmol/L)显著促进睾丸间质细胞分泌睾酮,但随着浓度的增加(5μmol/L),大豆苷元和染料木素均显著抑制Leydig细胞的睾酮分泌。

Objective： To investigate the effects of phytoestrogens （daidzein and genistein ） on the testosterone production of rat Leydig cells and the possible mechanisms. Methods ： Primary Leydig cells were obtained from 3-month old male SD rats using discontinuous Percoll density gradient centrifugation. The effects of phytoestrogens at various concentrations were evaluated by ELISA, with hCG as the positive control. The mRNA expression of P450 side-chain cleavage enzyme （P450scc） was analyzed by semi-quantitative RT-PCR. Results： Genistein at 0. 1 μmol/L obviously promoted the secretion of testosterone and upregulated the mRNA level of P450scc. At a higher concentration of 5 μmol/L, however, both daidzein and genistein significantly inhibited the testosterone production of Leydig cells （P 〉 0. 05）. Conclusion ： Genistein can promote the testosterone production of Leydig cells at a low concentration （0.1 μmol/L）, but both daidzein and genistein can inhibit it at a higher concentration （ 〉 5 μmol/L）.