中西医结合治疗原发性胆汁性肝硬化小鼠疗效分析

Analysis on the therapeutic effect of the integrated treatment with Chinese and western medicine for primary biliary cirrhosis in mice

探讨熊去氧胆酸(UDCA)和"一贯煎"在原发性胆汁性肝硬化小鼠中的疗效。对polyI∶C注射的C57BL/6雌性小鼠分别给予UDCA、"一贯煎"和两种药物联合治疗,分析不同药物治疗组血清ALP、-γGT的变化,实时荧光定量RT-PCR方法检测脾脏单个核细胞BCMA mRNA的表达,并分析肝门管区单个核细胞浸润率。结果表明,PBC模型鼠肝门管区单个核细胞浸润率、脾脏单个核细胞BCMA mRNA的表达较正常小鼠明显增加(P<0.05);不同治疗组较模型对照组ALP、-γGT显著下降(P<0.05),"一贯煎"或联合治疗还能减少肝门管区单个核细胞浸润率、下调脾脏单个核细胞BCMAmRNA和ALP。"一贯煎"或联合熊去氧胆酸治疗PBC小鼠疗效更好,为以后中西医联合治疗PBC提供了理论依据和技术支持。

To investigate the therapeutic effect of ursdeoxycholic acid （UDCA） and the Chinese medicine YIGUANJIAN for the treatment of primary biliary cirrhosis （PBC） in mice, UDCA and YTGUANJIAN were given to female C57BL/6 injected with poly I： C separately or in combination, and the changes in serum ALP and γ-GT of different groups with treatment of mice were analyzed. Meanwhile, real time fluorescent quantitative RT-PCR assay （FQ-RT-PCR） was used to detect the mRNA expression of the B cell maturation antigen （BCMA） on mononuclear cells in spleen and the rate of infiltration of this kind of cells in the portal region of liver was analyzed. It was demonstrated that the infiltrative rates of mononuclear cells in the portal region of liver and the BCMA mRNA expression on mononuelear cells in spleen were higher than those of the control mice （P〈0.05）. Also, the serum level of ALP and γ-GT of different groups with treatment of mice was markedly reduced （P〈0.05）. YIGUANJIAN or its combined treatment with UDCA could reduce the rate of infiltration of mononuclear cells in the portal region of liver and down-regulate the BCMA mRNA and ALP in mononuclear cells of spleen. It is concluded that use of YIGUANJIAN or the combined therapy with UDCA for the treatment of primary biliary cirrhosis in mice is better than the single use of UDCA alone. These results would provide theoretic basis for the integrated treatment with Chinese and western medicine to treat cirrhosis.