TLR9 mRNA在实验变态反应性神经炎中表达的研究

The expression of TLR9 mRNA in Lewis rats with experimental allergic neuritis

观察TLR9在实验性变态反应性神经炎（EAN）大鼠的坐骨神经、脾脏、外周血、淋巴结的动态表达,探讨TLR9与EAN的发病关系,为临床寻找新的治疗自身免疫性疾病的策略奠定理论基础。将36只Lewis大鼠随机分为抗原注射组（EAN组：n=4,4,4,4）,完全弗氏佐剂组（CFA组：n=4,4,4,4）,生理盐水对照组（NS组：n=4）,EAN组用抗原乳剂（100μg P0 180~199,100μl完全弗氏佐剂,100μl生理盐水）进行免疫,观察免疫后大鼠的发病情况和组织病理学改变。分别在第7天、第16天、第24天,第33天处死动物,取坐骨神经根、脾脏、外周血和淋巴结,制作病理切片行HE染色和Weil染色观察其病理改变,利用RT-PCR检测TLR9的mRNA表达水平。结果：EAN大鼠在发病16 d时症状达高峰,第33天时好转,TLR9 mRNA整个实验过程中一直呈上升趋势,前后各时间点相比具有统计学意义（P〈0.05）,与对照组相比有显著差异。结论：TLR9在EAN的起始及效应阶段有着重要作用。

The expression of TLR9 mRNA in Lewis ears with experimental allergic neuritis （EAN） was observed in order to get some idea for the treatment of Guillain-Barre syndrome （GBS） and other autoimmune disorders, in which male Lewis rats were immunized with 100 micrograms of component of PNS myelin sheath protein P0 188 199 and complete Freund＇s adjuvant （CFA） and the clinic signs and pathological changes in the sciatic nerves of rats were observed. Rats immunized and the control rats were sacrificed at 7, 16, 24 and 33 days after immunization and the histopathological changes occurring in the embedded paraffin sections of sciatic nerves of rats were examined with HE and Weil staining. Meanwhile, the expression levels of TLR9 mRNA in spleens, sciatic nerves, peripheral blood and lymph nodes were detected dynamically by RT-PCR assay. The experimental results showed that the rats with EAN got the peak of clinic score at 16 days post-immunization, while the clinic manifestations ameliorated obviously 33 days after immunization. In addition, the expression of TLR9 mRNA was upregulated in the whole course of experimentation and it was comparatively higher than that of the control eats with significant difference （P〈20.05）. These results suggest that TLR9 may play an important role in the initial and effecting stages in pathogenesis of experimental allergic neuritis.