17β-E_2对子宫内膜癌细胞增殖及其p-Erk表达的影响

Effect of 17β-E_2 on proliferation and expression of p-Erk in endometrial carcinoma cells

目的探讨17β-雌二醇（E2）对人子宫内膜腺癌Ishikawa和HEC-1A细胞增殖、周期及其p-Erk表达的影响。方法用MTT法、流式细胞术检测不同浓度17β-E2作用Ishikawa和HEC-1A不同时间后的细胞吸光度值及细胞周期,免疫细胞化学法检测细胞的p-Erk。结果随17β-E2浓度增加,Ishikawa细胞吸光度值上升,并呈时间依赖性（P〈0.01）,G0-G1期细胞比例下降（P〈0.01）,S期比例升高（P〈0.01）;HEC-1A细胞吸光度值及细胞周期无明显变化（P均〉0.05）。10-6mol/L的17β-E2作用于Ishikawa细胞30 min、作用于HEC-1A细胞15 min时,其p-Erk表达最强。结论17β-E2可以促进Ishikawa细胞的增殖和周期进展,而且可以通过非转录效应迅速激活Ishikawa和HEC-1A中MAPK信号转导通路。

Objective To observe the influence of 17β-estradiol （E2） on proliferation, cell cycle progression, expressions of p-Erk in endometrial carcinoma cells Ishikawa and HEC-1A.Methods 17β-E2 was used to Ishikawa and HEC-1A cells,the pmliferation and cell cycle of Ishikawa and HEC-1A were detection by MTT and fluorescence-activated cell sorting technique, the p-Erk were examined by immunoprecipitation in Ishikawa and HEC-1A cell after stimulated with 10-6 mol/L 17β-E2 for different times. Results With increased concentrations of 17β-E2, the proliferation of Ishikawa cell increased gradually and in a time-dependent manner（ P 〈 0.01） ,percentage of Ishikawa cell at Go - G1 phase decreased （ P 〈 0.01） and percentage of S phase cells increased significantly（ P 〈 0.01 ）, whereas that of HEC-1A cell didn＇t show significant alteration.The maximal activation of p-Erk took place at 30 min in Ishikawa cell and 15 rain in HEC-1A cell after stimulation with 10^-6 mol/L 17β-E2 .Conclusion 17β-E2 not only promote the cell proliferation and the cell cycle distrubion of endometrial carcinoma cell lines, but also activate promptly MAPK signaling pathway by non-nuclear actions in endometrial carcinoma cells.