RNAi技术沉默Bcl-xL基因表达对食管癌细胞增殖和侵袭的影响

Effect of RNAi silenced Bcl-xL gene on proliferation and invasion ability of human esophageal cancer cell

目的探讨RNA干扰(RNAi)技术沉默Bcl-xL基因表达对食管癌细胞增殖和侵袭能力的影响。方法将Bcl-xL小干扰RNA(siRNA)转染食管癌EC109细胞,分别采用荧光实时定量RT-PCR和Western blot方法检测食管癌细胞Bcl-xL mRNA和蛋白,采用软琼脂集落培养试验和Boyden小室模型检测癌细胞增殖和侵袭力。结果与对照组相比,siRNA转染组细胞Bcl-xL mRNA和蛋白水平明显下调(P均<0.05),所形成的软琼脂集落数和穿膜细胞数明显减少(P均<0.05),并均呈浓度依赖性。结论采用RNAi技术沉默Bcl-xL基因表达,可抑制食管癌细胞的增殖和侵袭能力。

Objective To study the effect of RNAi silenced Bcl-xL gene on invasion and proliferation of human esophageal cancer cell.Methods Esophageal cancer cell line ECI09 were transfected with different dose of Bcl-xL siRNA. The Bel-xL mRNA and protein were detected by real time RT-PCR and Western blot, respectively. The anchorage-independent proliferation of cancer cells were determined by colony formation in soft agar. The invasion ability of esophageal cancer cells were evaluated by boyden chamber model.Results The Bcl-xL mRNA and protein of tranfected cell was inhibited signifcanfly in a dose-dependent manner （ P 〈 0.05）. Compared with control group, both colony formation in soft agar and cells traversed membrane of transfected cells re- duced significantly in dose-dependent manners,resepectively.Conclusion Silencing Bcl-xL expression by RNAi may inhibit proliferation and invasion ability of esophageal cancer cell.