宫颈鳞状细胞癌组织中PTEN、PCNA的表达及意义

Expression and significance of PTEN and PCNA in cervical squamous carcinoma tissue

目的检测酪氨酸磷酸酶蛋白(PTEN)、增殖细胞核抗原(PCNA)的表达并探讨其意义。方法采用免疫组化S-P法检测70例宫颈鳞状细胞癌组织中PTEN、PCNA表达水平,并以11例正常宫颈组织为对照。结果与正常宫颈组织相比,宫颈鳞状细胞癌PTEN的阳性表达率明显降低,而PCNA阳性表达率明显增高(P均<0.05)。PTEN的表达与PCNA的表达呈负相关(r=-0.574,P<0.01)。PTEN、PCNA阳性表达率与宫颈鳞癌组织学分级、临床分期和淋巴结转移有相关性(P均<0.05)。结论PCNA的异常高表达与PTEN的异常低表达参与了宫颈癌的发生、发展过程。联合检测PTEN、PCNA蛋白对宫颈鳞癌临床诊断、确定治疗方案及预后评估有一定意义。

Objective To detect the expression and the significance of PTEN and PCNA in cervical squamous carcinoma. Methods PTEN and PCNA expression were detected by immunohistochemistry method in 70 cases of cervical squamous carcinoma and 11 cases of normal cervical specimens. Results Compared with normal cervical tissue, the positive expression rate of PTEN increased and PCNA reduced in cerrical squamous carcinoma. The expression of PTEN and PCNA was negatively related （r=-0.574, P〈0.01）. There were significant correlation between the expression of PTEN, PCNA and the histological grade, clinical stage, lymph node metastasis of cervical squamous carcinoma. Conclusion The abnormal high expression of PCNA and low expression of PTEN participate the pathogenesis of cervical carcinoma. PTEN and PCNA protein can be used in clinical diagnose , treatment and prognosis evaluate of cervical squamous carcinoma.