摘要
目的检测开角型青光眼患者房水中基质金属蛋白酶(MMP)-3及金属蛋白酶组织抑制因子(TIMP)-1的表达,并探讨其意义。方法采用酶联免疫反应(ELISA)双抗体夹心法检测开角型青光眼(A组)和年龄相关性白内障(B组)患者房水中MMP-3和TIMP-1的水平。结果A、B两组MMP-3水平分别为(68.0310±4.1400)、(69.7941±2.1315)μmol/L;TIMP-1水平分别为(6.7604±0.6632)、(3.1617±0.1603)μmol/L,MMP-3/TIMP-1分别为9.9017±0.3447、22.0592±1.0172,以上指标两组相比,P均<0.05。结论开角型青光眼患者房水中MMP-3表达,TIMP-1表达升高,二者共同促进细胞外基质的异常堆积从而参与了开角型青光眼的发病机制。
Objective To detect the expression of matrix metalloproteinase （MMP）-3 and MMP tissue inhibitor （TIMP） -1 in the aqueous humor of open-angle-glaucoma （POAG） and age-related-cataract （ ARC ） patients. Method MMP-3 and TIMP-1 levels in aqueous humor samples from patients with POAG（ A group） and ARC（ B group） were detected by enzyme-linked immunosorbent assay （ELISA）. Results The MMP-3 levelsin A, B group were （68. 0310 ±4.1400）,（69.7942 ±2. 1352） μmol/L; the TIMP-1 levels were（6. 7604±0. 6632） ,（3. 1617 ±0. 1603） μmol/L, MMP-3/TIMP-1 were 9. 9017 ±0. 3447,22. 0592 ± 1. 0172. There were significant difference in A,B groups（ P 〈 0.05 ）. Conclusions The expression of MMP-3 decreases and TIMP-1 upgrades in aqueous humor of POAG patients. They both encourage the paraplasm of extraeellutar matrix and participate the genesis of POAG.
出处
《山东医药》
CAS
北大核心
2008年第47期22-23,共2页
Shandong Medical Journal
基金
山东省自然科学基金资助(Y2002C32)
