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胰岛β细胞游离脂肪酸受体1表达对胰岛素分泌的影响 被引量：1

Effect of the expression of free fatty acid receptor 1 on the insulin secretion of β cells
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摘要 目的研究高脂、罗格列酮和非诺贝特对体外培养的β细胞瘤细胞系NIT-1细胞游离脂肪酸受体1(FFAR1)mRNA表达和胰岛素分泌功能的影响。方法将NIT-1细胞随机分为正常组(NC组)、高脂组(PA组)、高脂加罗格列酮组(RG组)、高脂加非诺贝特组(FF组)体外培养48 h,用放免法检测细胞基础胰岛素分泌(BIS)及葡萄糖刺激的胰岛素分泌(GSIS)水平,酶法检测胞内甘油三酯(TG)含量,RT-PCR技术检测细胞内FFAR1 mR-NA水平。结果与NC组比较,PA组GSIS降低,细胞内TG含量增多,FFAR1 mRNA表达明显上调。与PA组比较,RG、FF组GSIS增强,细胞内TG含量减少;RG组FFAR1 mRNA表达与之接近,FF组则明显减少,接近NC组水平。结论高脂对β细胞的毒性作用部分是通过上调FFAR1 mRNA表达所致;非诺贝特可通过抑制这种作用保护β细胞功能;罗格列酮对β细胞功能的保护作用与之无关。 Objective To investigate the effect of palmitic acid, rosiglitazone and fenofibrate on the expression of free fatty acid receptor 1 （ FFAR1 ） and the insulin secretion function of NIT-1 cells were cultured in vitro. Methods NIT-I cells were divided into four groups, nonmal group （NC group）, palmitic acid group （PA group）, palmitic acid plus rosiglitazone group （ RG group） and palmitic acid plus fenofibrate group （ FF group）, then cultured respectively for 48 h in vitro. The levels of insulin secretion were detected with radioimmunoassay, intracellular tfiglycerides contents were determined by enzyme immunoassay and FFAR1 mRNA levels were detected by RT-PCR. Results Compared to the NC group, the GSIS in the PA group decreased, while the level of TG and expression of FFAR1 mRNA increased. Compared o the PA group, GSIS in RG and FF group increased, TG decreased, while the expression of FFAR1 mRNA in FF group decreased too. Conclusions It is one of the mechanisms of lipotoxicity to upregulate the expression of FFAR1 mRNA, and fenofibrate can inhibit it. Rosiglitazone can protect the function of beta cells, but it has nothing to do with the mechanisms above.
作者 田源 陈璐璐 王咏波 李燕
机构地区 华中科技大学同济医院附属协和医院 襄樊市中心医院
出处 《山东医药》 CAS 北大核心 2008年第48期15-17,共3页 Shandong Medical Journal
基金 湖北省自然科学基金资助项目(2003ABA143)
关键词 游离脂肪酸受体1 棕榈酸 罗格列酮 非诺贝特 胰岛素分泌 甘油三酯 free fatty acid receptor 1 palmitie acid rosiglitazone fenofibrate insulin secretion triglyeride
分类号 R587.1 [医药卫生—内分泌]
  • 相关文献

参考文献9

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二级参考文献12

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共引文献37

同被引文献20

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山东医药
2008年 第48期

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