摘要
研究了蚕丝固定化葡萄糖氧化酶的工艺条件和稳定性。结果表明:蚕丝与对-β硫酸酯乙砜基苯胺(SESA)进行反应的最适条件是pH=10.8,SESA:蚕丝=1.9:1(W/W)。生成的对氨基苯磺酰乙基蚕丝(ABSE─蚕丝)经重氮化后与葡萄糖氧化酶偶联的最适条件是:pH=7.5,偶联时间>12h。加酶量为70-110U/g蚕丝时,所得固定化脂肪酶活力为35~45U/g蚕丝,此时固定化酶的活力回收率较高(>40%)。固定化葡萄糖氧化酶稳定性较高,其操作半衰期约为21d。
The conditions of the glucose oxidase (GOD) immobilized on the silk have been studied, The results showed that the optimal conditions of the reaction between Silk and p-β-sulfuric acid ester ethyl sulfon aniline (SESA) are pH = 10. 8 and SESA: Silk = 1. 9: 1 (w/w). The product P--aminobenzent sulfaniliyl ethyl Silk (ABSE --Silk)was diazotized and linked with GOD. The optimal pH for glucose linked to diao -compound is 7. 0. The link time is at last 12h. When the amount Of GOD is 70 -110u/ g (Silk), the activity of immobilized COD is 35 -45u/ g (Silk) and the recovery rate of the enzyme is above 40%. The immobilized GOD is fairly stability, its operational half life is about 21 d.
出处
《食品科学》
EI
CAS
CSCD
北大核心
1998年第4期3-6,共4页
Food Science