蚕丝固定化葡萄糖氧化酶的研究

Immobilization of Glucose Oxidase on the silk

研究了蚕丝固定化葡萄糖氧化酶的工艺条件和稳定性。结果表明：蚕丝与对-β硫酸酯乙砜基苯胺（SESA）进行反应的最适条件是pH＝10．8，SESA：蚕丝＝1．9：1（W／W）。生成的对氨基苯磺酰乙基蚕丝（ABSE─蚕丝）经重氮化后与葡萄糖氧化酶偶联的最适条件是：pH＝7．5，偶联时间＞12h。加酶量为70－110U／g蚕丝时，所得固定化脂肪酶活力为35～45U／g蚕丝，此时固定化酶的活力回收率较高（＞40％）。固定化葡萄糖氧化酶稳定性较高，其操作半衰期约为21d。

The conditions of the glucose oxidase (GOD) immobilized on the silk have been studied, The results showed that the optimal conditions of the reaction between Silk and p-β-sulfuric acid ester ethyl sulfon aniline (SESA) are pH = 10. 8 and SESA: Silk = 1. 9: 1 (w/w). The product P--aminobenzent sulfaniliyl ethyl Silk (ABSE --Silk)was diazotized and linked with GOD. The optimal pH for glucose linked to diao -compound is 7. 0. The link time is at last 12h. When the amount Of GOD is 70 -110u/ g (Silk), the activity of immobilized COD is 35 -45u/ g (Silk) and the recovery rate of the enzyme is above 40%. The immobilized GOD is fairly stability, its operational half life is about 21 d.