摘要
研究了采用OPA柱前衍生法测定西红柿中的谷胱甘肽。通过ODSC─18柱分离，流动相为60％的0．15mol／L乙酸钠溶液（pH7．0）和40％的甲醇。荧光检测器波长为：EX：340nm和EM：420nm。回收率为99．78－99．87％，变异系数（CV）为1．19％。
A method for measurement of glutathione in tomato by OPA pre -- column deriration is described . The sample is treated simply through shivering, oscillating, centrifuging, deriration and separated by DOS C-- 18 columm. The mobile phase is 60% 0. 15mol/ L sodium acetdate (pH7. 0). The fluorescence detector wavelength is EX 340 nm and EM 420nm and its recorery is from 99. 78 to 99. 87%. Coefficient of variation is 1. 19% .
出处
《食品科学》
EI
CAS
CSCD
北大核心
1998年第4期52-55,共4页
Food Science
关键词
邻苯二甲醛
柱前衍生
西红柿
谷胱甘肽
orthophthaladetyde Pre -- column deriation Tomato glutathione
