鹅观草属种子消毒及无菌播种的研究

Study on Roegneria Seed Sterilization and Aseptic Seeding

研究消毒剂、处理时间对中间鹅观草种子(Roegneria sinica var.media)灭菌效果的影响和培养基对肃草(Roegneria stricta f.stricta)、偏穗鹅观草(Roegneria turczaninoviivar.macrathera)、大芒鹅观草(Roegneria komarovii)种子萌发的影响。结果表明:0.1%氯化汞处理3 min,种子污染率为0,萌发率达96.67%;3种鹅观草种子在适合培养基中的萌发情况是偏穗鹅观草>肃草>大芒鹅观草,适合培养基分别是1/2 MS、MS、MS。

Study the sterilization effect of sterilants and treat time on Roegneria sinica var. media and the influence of substrate to seed germination of Roegneria stricta f. stricta, Roegneria tarczaninovii var. macrathera and Roegneria komarovii. The result showed that the germination percentage was 96.67% on condition that 0.1% HgCl2 immersing seeds, and the pollution percentage of seeds was zero. The suitable substrance of the three Roegneria sirtica var. media seed were seporiate 1/2 MS, MS and MS. and their germination manifest on their suitable substrate in squence Roegneria turczaninovii vat. macrathera, Roegneria stricta f. stricta and Roegneria komarovii.