不同含水量的紫云英种子库存20年后活力研究

Research Astragalus Sinicus Seed Vigor with Different Water Content Conserved in the Gene Bank for 20 Years

为了探讨种子长期贮藏的最适含水量,较为全面评价种子长期保存后的活力,将紫云英种子(初始含水量为9.63%)于35℃下加温干燥,使种子含水量分别降至8.36%、7.74%、6.92%、6.47%和5.97%后经密封包装藏于-1℃种质库条件下进行长期保存,20年后取出测定它们的种子萌发能力、田间生长能力、电导率等。结果表明:经20年保存后,含水量为8.36%、7.74%、6.92%和6.47%的种子发芽率与贮藏前相比差异极显著,发芽率下降明显不适宜长期保存,含水量5.97%的种子发芽率与贮藏前相比差异不明显,活力最高,适合低温长期保存。此研究为绿肥种质资源长期安全保存积累了科学的数据,对指导种质保存具有重要的现实意义。

In order to discuss the most suitable water content for long time conservstion, and comprehensive appraise vigor after long-term preservation . This experimental used Astragalus Sinicus seed＇ s with 9.63% water content as material ,dried in 35℃ to decrease water content to 8.36%, 7.74%, 6.92%, 6.47% and 5.97%, and packed after seal Tibet to carry under - 1℃ gene bank condition long-time preservation, after 20 years , took out and determined their germination ability, field growth ability, the conductivity and so on. The result shows： After 20 years preservation, the germination rate which with water content 8.36% and 7.74%, 6.92% and 6.47% were extremely remarkable compare to before 20 years,suggested this type seeds were not suitable long-time preservation;The germination rate which with 5.97% water content were not obviously ,suggested this type seeds were suitable long-tiome preservation under low temperature. This result could provide scientific data for the manure germplasm resources long-term safety save and has important realistic significance for preserving germplasm.