乙型肝炎患者血清HBV外膜大蛋白与HBV-DNA检测的相关性及临床意义

The correlation of HBV outer membrane large protein and HBV-DNA and its clinical significance

目的通过乙型肝炎患者血清HBV外膜大蛋白(HBV-LP)和HBV-DNA的检测,探讨两者在判断乙型肝炎病情和HBV复制时的相关性及其临床意义。方法采用荧光定量PCR方法对乙型肝炎患者血清的HBV-DNA进行检测,采用酶联免疫吸附试验(ELISA),对上述标本进行HBV-LP和HBV血清免疫学标志物(HBV-M)模式的检测。结果 HBV-LP的检出率与HBV-DNA的检出率差异有统计学意义(P<0.05),不同HBV-M模式的HBV-DNA与HBV-LP检出结果差异有统计学意义(P<0.05),而HBV-DNA拷贝数对数值与HBV-LP的表达显著相关(r=0.677,P<0.01)。结论血清中HBV-LP的含量与HBV-DNA拷贝数具有较好相关性,但HBV-LP尚不能完全代替HBV-DNA的检测来反映HBV复制情况。

Objective To detect HBV outer membrane large protein（HBV-LP） and HBV- DNA, and to explore their correlation in judging the pathogenetic condition and HBV replication, as well as its clinical significance. Methods Serum level of HBV-DNA was quantitatively detected by using real-time polymerase chain reaction, and while HBV-LP and HBV markers were measured with enzyme-linked immunosorbent assay（ELISA） in infected sera from 238 patients with hepatitis B. Results There was significant difference of detection rate between HBV DNA and HBV-LP（P〈0.05）. The expression of HBV-DNA and HBV-LP had significantly difference among various patterns of HBV serological markers（P〈0.05）. HBV-LP expression was markedly correlated with the logarithm of HBV-DNA level（r=0. 677,P〈0.01）. Conclusion There is a prefect correlation between the copies of HBV-DNA and the level of HBV-LP, but HBV-LP expression can not still reflect the status of HBV replication.