温度对ACD、CPDA全血ATP作用的研究

Effects of temperature on ATP of ACDblood and CPDAblood during blood preservation

目的：研究非４°条件ＡＣＤ、ＣＰＤＡ全血保存过程中ＡＴＰ变化特点。方法：荧光素－荧光素酶法。结果：４°Ｃ、２２°Ｃ、２８°Ｃ、３３°Ｃ条件下ＡＣＤ、ＣＰＤＡ全血在保存过程中ＡＴＰ变化随温度的升高而加剧，非４°Ｃ条件下同一温度组保存１ｄ后ＣＰＤＡ组与ＡＣＤ组差异有显著性意义。结论：以ＡＴＰ值大于１．５μｍｏｌ／ｇＨｂ为临界，提出了血液保存的有效期限。

Objective:To study the ATP of ACD and CPDAblood during the preservation under non4°C conditions.Methods:Measure the value of ATP by fluorophotometry.Results:The value of ATP changed at 4°C was compared with those at 22°C,28°C and 33°C during blood preservation. CPDAblood containing adenine maintained higher ATP than ACDblood at above temperatures.Conclusion:According to the permitted lowest limit of ATP (1.5 umol/gHb), we found the new RBC survival periods under 22°C,28°C,33°C.And the CPDAblood may maintain higher ATP than ACDblood at above temperatures.