大菱鲆对迟缓爱德华氏菌的免疫应答

Immune Response to Edwardsiella tarda in Turbot (Scophthal musmaximus)

用制备的迟缓爱德华氏菌灭活全菌和主要外膜蛋白(OMP)对大菱鲆进行腹腔注射及肛门灌注免疫,以Ig+细胞数量及血清抗体效价为指标,评价和比较大菱鲆对不同抗原的免疫应答水平。结果显示,注射2种抗原后,外周血中Ig+细胞数量及血清抗体效价均呈明显增加趋势,注射OMP大菱鲆的外周血Ig+细胞数量及血清抗体效价不显著低于注射灭活全菌大菱鲆(外周血Ig+细胞:P=0.390;抗体效价:P=0.300);肛门灌注2种抗原后,大菱鲆外周血和后肠中Ig+细胞数量及血清凝集效价亦均呈增加趋势,灌注OMP大菱鲆后肠、外周血中Ig+细胞数量和血清抗体效价均不显著高于灌注灭活全菌大菱鲆(外周血Ig+细胞:P=0.056;后肠Ig+细胞:P=0.163;抗体效价:P=0.195)。结果表明,注射和肛门灌注迟缓爱德华氏菌灭活全菌和OMP均能够诱导大菱鲆产生针对迟缓爱德华氏菌的特异性免疫应答。本文结果对鱼类疫苗的研制具有参考价值。

The number of Ig ＋ cells as well as serum agglutination titer was measured in unvaccinated turbot, vaccinated ones by inactivated E. tarda and OMP via intraperitoneal injection （i. p. ） and anal incubation （a. i. ）. The results are summarized as follows： After immunization with inactivated E. tarda and major OMP via i. p., the number of Ig ＋ cells in PBL and the plasma agglutination titer respectively presented an increasing trend in vaccinated turbot, and they were insignificantly lower in turbot vaccinated by OMP than those vaccinated by inactivated E. tarda （number of Ig ＋ cell in PBL： P = 0.39; agglutination titer： P = 0.30） . After immunization with inactivated E. tarda and major OMP via a. i., the number of Ig ＋ cell in gut and in PBL as well as the plasma agglutination titer respectively presented an increasing trend in vaccinated turbot, and they were insignificantly lower in turbot vaccinated by inactivated E. tarda than those vaccinated by major OMP（number of Ig ＋ cell in PBL： P = 0. 056 ; number of Ig ＋ cell in gut ： P = 0. 163 ; agglutination titer： P = 0. 195）. These results show that the inactivated E. tarda and major OMP both can induce specific immune response against E. tarda in turbot after administration of i.p. and a. i.. These are essential for designing vaccination strategies in fish.