纳米微反应器化学发光法测定氢溴酸加兰他敏

Chemiluminescence Analysis of Galantamine Hydrobromide with a Nanometer Microreactor

在酸性条件下，氢溴酸加兰他敏被质子化后与AuCl4^-形成离子缔合物被二氯甲烷所萃取，当缔舍物被带入含鲁米诺的氯化十六烷基三甲基铵（Cetyltrimethylammonium Chlorine，CTAC）逆胶束纳米微反应器中时，离解出来的AuCU与鲁米诺产生化学发光．发光强度与氢溴酸加兰他敏的含量呈线性关系，从而可间接测定氯溴酸加兰他敏的含量．在优化的试验条件下，线性范围为0．001～15μg／mL，检出限（3σ）为0．05ng／mL，对浓度为1．0μg／mL的氢溴酸加兰他敏进行11次平行测定，相对标准偏差（RSD）为2．24％．该法已成功用于片剂、针剂和生物体液中氢溴酸加兰他敏的测定．

Under the acidic condition, galantamine hydrobromide was protonated and formed an ion-complex with the negative ion AuCl4^-, and the ion-complex was extracted by dichloromethane. When the ioncomplex entered a reversed micellar nanometer microreactor of cetyhrimethylammonium chlorine containing luminol, the dissociated AuCl4^- reacted with luminol and produced an analytical chemiluminescnece signal. Under the optimum conditions, the CL response was linear to galantamine hydrobromide concentration ranging from 0. 001 to 15 g/mL and the limit of detection was 0.05 ng/mL with a relative standard deviation （n=11） of 2.24% for 1.0 g/mL galantamine hydrobromide. The method has been successfully applied to the determination of the studied drug in tablets, injections and biological fluids.