配合物[RE2(H2L)2(HL)2(2，6-H2PDA)(H2O)2]·2H2O的合成、晶体结构及其与DNA作用方式初探

Synthesis, Crystal Structure and Preliminary DNA-binding Studies of [RE_2(H_2L)2_(HL)_2(2,6-H_2PDA)(H_2O)_2]·2H_2O

以N-(2-异丙酸)-邻羟基苯甲酰腙(C10H10N2O4,H3L)、2,6-吡啶二甲酸(2,6-H2PDA)与RE(NO3)3·nH2O(RE=Pr,Eu)在室温下反应,合成了配合物1[Pr2(H2L)2(HL)2(2,6-H2PDA)(H2O)2]·2H2O和配合物2[Eu2(H2L)2(HL)2(2,6-H2PDA)-(H2O)2]·2H2O,对其进行了元素分析、红外光谱、紫外光谱等表征,测定了两种配合物的晶体结构.通过紫外吸收光谱、荧光发射光谱和稳态荧光猝灭方法及其与溴化乙锭(EB)的竞争实验研究了两种配合物与小牛胸腺DNA的作用情况.结果表明,两种配合物与小牛胸腺DNA均是以插入方式结合的.

Complex 1 [Pr2（H2L）2（HL）2（2,6-H2PDA）（H2O）2]·2H2O and complex 2 [Eu2（H2L）2（HL）2（2,6- H2PDA）（H2O）2]·2H2O were obtained by reactions of N-（2-propionic acid）-salicyloyl hydrazone （C10H10N2O4, H3L）, 2,6-pyridinedicarboxylic acid （2,6-H2PDA） and RE（NO3）3·nH2O （RE=Pr, Eu） at room temperature, and characterized by elemental analyses, IR, UV, and single crystal X-ray diffractometry. In addition, the DNA binding properties of the two complexes were studied by means of UV absorption spectrum, emission spectroscopic spectrum, steady-state emission quenching and DNA competitive binding with ethidium bromide （EB）. The results indicated that the two complexes bound to calf-thymus DNA in an intercalative mode.