利用同源菌株融合改良农抗5102产生菌 Ⅲ.融合子产生新活性物质的分离、精制和初步鉴别

STRAIN IMPROVEMENT OF ANTIBIOTIC 5102 PRODUCING Streptomyces hygroscopicus var. yinchengensis BY PROTOPLAST FUSION BETWEEN HOMOGENOUS STRAINS Ⅲ. ISOLATION, REFINEMENT AND PRIMARY IDENTIFICATION OF THE NEW ACTIVE SUBSTANCE FROM THE FUSANT SR 7

农抗５１０２产生菌同源菌株９０１１和ＦＲ００８的原生质体融合重组子ＳＲ７能产生１种两亲本所没有的抗细菌活性物质。该物质可用７２４弱酸性阳离子树脂从发酵滤液中吸附，以７％硫酸解吸后，经７１７强碱性阳离子树脂柱层析精制。经热稳定性、酸碱稳定性、纸电泳、纸层析谱、紫外吸收光谱和呈色反应等多项试验检测，初步判定为１种强碱性水溶性氨基糖苷类抗生素。它具有广谱抗Ｇ＋、Ｇ－细菌的活性，对各种真菌和酵母无活性。

The fusion recombinant SR 7 of the antibiotic 5102 producing homogenous strains 90 11 and FR 008 produced a new antibacterial substance the parents could not. This active substance can be adsorbed by weak acidic cation exchange resion (Amberlite IRC 50), desorbed with 7% sulphuric acid and finally refined by volume chromatography, UV absorption spectrum and color reaction indicate that the antibacterial substance of SR 7 is likely a aminoglycoside with a wide range antibacterial spectrum including both gram positive and negative bacteria, but is inactive against fungi and yeasts.