紫外线对柑桔原生质体的影响初探

PRELIMINARY STUDY ON EFFECTS OF UV ON CITRUS PROTOPLASTS

用强度约为５６μＷ／ｍｍ２的紫外线ＵＶ分别照射Ｐａｇｅ和Ｍｕｒｃｏｔ原生质体３０ｓ、１ｍｉｎ和５ｍｉｎ，发现随着辐照时间的延长，原生质体活力趋于下降。Ｍｕｒｃｏｔ的原生质体较Ｐａｇｅ的原生质体对ＵＶ敏感。辐射１ｍｉｎ和５ｍｉｎ的原生质体在培养过程中未能出现分裂，而是慢慢地发生质壁分离，最后破裂。而辐射３０ｓ的原生质体与对照都恢复分裂形成细胞团并长出愈伤组织。

UV,with the intensity of 56 μW/mm 2,was utilized to irradiate the protoplasts of Page tangelo(C.reticulata×C.paradisi cv. page )and Murcott tangor(C.reticulata×C.sinensis cv. murcott ).The result demonstrated that viabilities of the protoplasts decreased with the increased irradiation time.Murcott was more sensitive to UV than Page.The protoplasts irradiated for 1 minute and 5 minutes did not divide.Instead,most of them experienced plasmolysis and broke. Nevertheless, those irradiated for 30 seconds divided and developed into small calli.