VA菌根真菌纯系增殖培养条件研究初报

STUDIES ON THE CONDITIONS FOR PROLIFERATING VESICULAR-ARBUSCULAR MYCORRHIZAL FUNGI

本试验研究了ｐＨ和磷素水平对ＶＡＭ菌剂接种势的影响。研究结果表明：在ｐＨ５～７范围内Ｇ．ｍｏｓｅａｅ的侵染能力和扩展能力随ｐＨ值的提高而增强，高磷时其产孢能力随ｐＨ值的提高而增强；磷素水平在３．５５～３５．５μｇ／ｇ范围内，高磷不利于Ｇ．ｍｏｓｅａｅ在寄主根内的扩展，高ｐＨ时侵染能力和产孢能力随磷素水平分别减弱和增强。增加ｐＨ值导致主根系生物量下降，高ｐＨ配合低磷水平能提高寄主根系生物量。其外接种后４周内的光照强度在很大程度上决定着ＶＡＭ真菌是否侵染成功。

Effects of pH and phosphorus on the inoculation potential of vesicular-arbuscular mycorrhizal inoculum were studied in this experiment.The results showed that within the range of pH 5 to 7,the infection ability and intracortex spread of G.mosseae increased,the sporulation ability incresed at high phosphorus when pH increased.Within the range of phosphorus 3.55μg/g to 35.5μg/g,the intracortex spread ability decreased,the infection ability decreased and sporulation ability increased at high pH when phosphorus increased.The biomass of host root system decreased when pH increased and the biomass increased at high pH low phosphorus.In addition,light intensity within 4 weeks after inoculation was a decisive factor if the VAM fungus could infect the host.