神经干细胞联合氯化锂对急性脊髓损伤修复的试验研究

Combination therapy of acute spinal cord injury of adult rat by neural stem cell and lithium chloride

目的:观察神经干细胞(neuralstemcells,NSCs)移植联合应用氯化锂(lithiumchloride,LiCl)对于大鼠急性脊髓损伤修复效果,并探讨二者相互作用。方法:利用MASCISimpacto(r多中心急性脊髓损伤打击器)制作大鼠脊髓打击伤模型,随机分为NSCs组、NSCs+LiCl组、LiCl组及空白对照组,分期进行BBB后肢运动功能评分及组织学检查,评价及比较各组修复效果。结果:NSCs组与NSCs+LiCl组BBB评分较LiCl组与对照组在2周后各时段差别有意义(P<0.01);NSCs+LiCl组BBB评分在4周后明显高于NSCs组(P<0.01);组织学检查表明NSCs组与NSCs+LiCl组组织学改变与LiCl组与对照组有显著差别,但是NSCs+LiCl组相对于NSCs组可以更明显的促进神经元再生。结论:NSCs联合LiCl具有良好的脊髓损伤修复作用,二者协同作用明显。

Objective： To explore the combination therapy effects of neural stem cell and lithium chloride （LiCL） on acute spinal cord injury of adult rat. Methods： The MASCIS system （multi-center acute spinal cord injury system） was taken to make spinal cord injury rat models. The animals were divided into 4 groups randomly： neural stem cell transplantation group （NSCs group）, neural stem cells and LiCl group（NSCs＋LiCl group）, lithium chloride treatment group （LiCl group） and the control group. The Basso-Beattie-Bresnahan （BBB） hindlimb motor function score and histological examination were carried out to evaluate repair effects of different groups. Results：The BBB scores of NSCs and NSCs＋LiCl groups were higher than that of LiCl and control group at the 2 weeks after operation （P〈0.01）; BBB scores of NSCs＋LiCl group at the 4 weeks was significantly higher than that of NSCs group （P〈0.01）. Comparing to the LiCl group and the control group, the histological differences of NSCs group and NSCs＋LiCl group was significant, however, NSCs＋LiCl group was better than other 3 groups in promoting neuron regeneration. Conclusion：It is more effective for functional and structural recovery of acute spinal cord injury adopting combination therapy of NSCs and LiCl.