视网膜母细胞瘤组织中热休克蛋白与Survivin表达关系及其对肿瘤细胞增生活性的影响

Relationship between heat shock proteins and Survivin in retinoblastoma cells and its effect on the proliferation of the cellular activity

目的探讨视网膜母细胞瘤（RB）组织中热休克蛋白（HSP）70、90的表达及其与凋亡调控因子Survivin表达关系，以及两者共同表达对RB细胞增生活性的影响。方法采用链霉素抗生物素蛋白-过氧化物酶免疫组织化学法检测43例RB及6例正常视网膜石蜡组织切片HSP70、HSP90、Survivin和Ki-67蛋白表达水平，以Ki-67蛋白标记指数作为肿瘤细胞增生活性标志，结合病理学特征分析HSPs-Survivin协同表达与肿瘤细胞增生活性关系。结果43例RB组织中HSP70阳性表达28例，占65．12％，HSP90阳性表达37例，占86．05％，Survivin阳性表达27例，占62．79％；6例正常视网膜组织中均无HSP70、HSP90及Survivin表达；HSP90阳性表达组中Survivin阳性表达率明显高于阴性表达组（P〈0．05）；HSP90阳性表达组及Survivin阳性表达组Ki-67蛋白标记指数均较表达阴性组明显增高（P〈0．05），HSP90-Survivin共同阳性表达组Ki-67蛋白标记指数较HSP90Survivin共同表达阴性或表达不一致组明显增高（P〈0．05）。但HSP70表达与Survivin相关性无统计学意义（P〉0．05），且对Ki-67蛋白标记指数无明显影响（P〉0．05）；HSPs、Survivin表达及Ki-67蛋白标记指数与RB组织学分型无关（P均〉0．05）。结论RB组织中HSP90与Survivin表达存在明显相关性，HSP90-Survivin共同阳性表达可能在RB细胞增生机制中具有重要意义。

Objective To investigate the relationship of the expression between heat shock protein （HSP） 70 and 90, and Survivin and its effects on the proliferative activity in retinoblastoma （RB） cells. Methods Expression of Survivin, HSPT0 and 90, and Ki-67 in conventional paraffin samples from 43 patients with RB and 6 healthy people was detected by streptavidin-biotin peroxidase （ SP ） immunohistochemieal method. Ki-67 labeling index was used to evaluate the proliferative activity in RB. Results In 43 cases of RB, positive expression of HSP70 and 90 and Survivin was found in 28 （65.12%）, 37 （86.05%） and 27 （62.79%） cases, respectively. None of the 6 normal retinal tissue expressed HSP70, HsP90 or Survivin. Positive expression of Survivin was more frequent in positive expressions of HsP90 than that in negative expressions of HSP90 （P〈0.05）. Ki-67 labeling index was higher in positive expressions of HSP90 and positive expressions of Survivin than that in their negative expressions respectively （P〈0.05）. Meanwhile, higher Ki-67 labeling index was found in positive HSP90-Survivin expressions than that in negative HSP90-Survivin expressions and those cases where only HSP90 or Survivin was found （P〈0.05）. Expression of HSP70 did not correlate with that of Survivin, nor had any significant effect on Ki-67 labeling index （ P〉 0.05）. Expression of HSPs and Survivin and Ki-67 labeling index did not correlate with histological types （P〉0.05）. Conclusion Expression of HsP90 correlates with that of Survivin in RB. Co-existence of Survivin and HSP90 probably plays an important role in the genesis of RB.